Shim-pack Velox C18

C18

Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a wide range of applications such as pharmaceutical, food, environmental and clinical and neutrals at moderately low and mid-range pH.

  • General purpose column for reversed-phase chromatography
  • Highest hydrophobic retention among Shim-pack Velox series
  • Compatible with moderately acidic to neutral mobile phases (pH 2-8)

Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment

The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 μm fully-porous ODS column described in the USP monograph is transferred to a new method with Shim-pack Velox C18 2.7 μm column, within USP allowable adjustments. Analytical time and solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.

Column

L/dp

Flow rate
(mL/min)

N

System suitability test result
(Requirement: %RSD < 2.0 %)

VP-ODS
(5 μm, 4.6 x 150 mm)

30,000

 

0.50

 

5,244

tR: 0.025 %
Area: 0.175 % (n=6)

Velox C18
(2.7 μm, 4.6 x 100 mm)

37,037
(+23 %)

0.50

9,497
(+81 %)

tR: 0.035 %
Area: 0.103 % (n=6)

0.93

4,466
(-15 %)

tR: 0.084 %
Area: 0.220 % (n=6)

For Research Use Only. Not for use in diagnostic procedures.

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Ordering Information

 Shim-pack Velox C18 Analytical Columns
 
Paticle Size (µm) Length (mm) / I.D. (mm) 2.1 3.0 4.6
1.8 30 227-32007-01 - -
50 227-32007-02 227-32008-01 -
100 227-32007-03 227-32008-02 -
150 227-32007-04 - -
2.7 30 227-32009-01 227-32010-01 227-32011-01
50 227-32009-02 227-32010-02 227-32011-02
100 227-32009-03 227-32010-03 227-32011-03
150 227-32009-04 227-32010-04 227-32011-04
5.0 50 - - 227-32012-01
100 - - 227-32012-02
150 - - 227-32012-03
250 - - 227-32012-04
 
 
 Shim-pack Velox EXP Guard Column Cartridge (3 pcs. per pack)
 
Paticle Size (µm) / I.D. (mm) 2.1 3.0 4.6
UHPLC 227-32031-01 227-32031-02 -
2.7 227-32032-01 227-32032-02 227-32032-03
5.0 - - 227-32033-01
 Shim-pack Velox EXP Cirect Connect Holder: 227-32041-01
 
 
 
 
Shim-pack Velox UHPLC Precolumn Filter (0.2 mm)
 
1 pc. per pack 227-32042-01
5 pcs. per pack 227-32042-02
10 pcs. per pack 227-32042-03
 

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